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SOLVED:As shown in this section, oligonucleotide synthesis features as the key step a tetrazole-catalyzed coupling of an alcohol (the first nucleotide) with a phosphoramidite (the second nucleotide). On first sight (focusing on all the nitrogen lone pairs), the function of tetrazole appears to be that of a base (to deprotonate the alcohol), but this heterocycle is actually quite acidic (p Ka=4.8), about as much as acetic acid (Section 19-4). Moreover, kinetic experiments indicate a ratedetermini
SOLVED:As shown in this section, oligonucleotide synthesis feature…
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