Solid-Phase Peptide Synthesis: The Merrifield Method - SlideServe

SOLVED:As shown in this section, oligonucleotide synthesis features as the key step a tetrazole-catalyzed coupling of an alcohol (the first nucleotide) with a phosphoramidite (the second nucleotide). On first sight (focusing on all the nitrogen lone pairs), the function of tetrazole appears to be that of a base (to deprotonate the alcohol), but this heterocycle is actually quite acidic (p Ka=4.8), about as much as acetic acid (Section 19-4). Moreover, kinetic experiments indicate a ratedetermini

Oligonucleotide Synthesis

Oligonucleotide Synthesis As shown in the scheme of Eigure 8-34... | Filo