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Solid-Phase Peptide Synthesis: The Merrifield Method - SlideServe
Solid-Phase Peptide Synthesis: The Merrifield Method. Solid-Phase Peptide Synthesis. In solid-phase synthesis, the starting material is bonded to an inert solid support. Reactants are added in solution.
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4.2K views · 56 reactions | DNA structure as art from Chromos / Coils of Living Synthesis with Andy Lomas, Mikhail Spivakov, The Babraham Institute and Jennifer Tividad (the vid shows predicted folding based on experiments showing points of contact between particular nucleotide sequences, active gene expression appears in red and chromosomes are labelled by colour at the end) | Max Cooper | Facebook
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Max Cooper
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Answered: You perform the following solid-phase oligonucleotide synthesis: DMT lodine, water, pyridine DMT-O acetic anhydride, trichioroacetic acid N-methylimidazole… | bartleby
bartleby.com
4 months ago
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SOLVED:As shown in this section, oligonucleotide synthesis features as the key step a tetrazole-catalyzed coupling of an alcohol (the first nucleotide) with a phosphoramidite (the second nucleotide). On first sight (focusing on all the nitrogen lone pairs), the function of tetrazole appears to be that of a base (to deprotonate the alcohol), but this heterocycle is actually quite acidic (p Ka=4.8), about as much as acetic acid (Section 19-4). Moreover, kinetic experiments indicate a ratedetermini
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